Composite

Part:BBa_K4011015:Design

Designed by: Peijia Lai   Group: iGEM21_LINKS_China   (2021-10-21)


pTac-RiboJ-TnaA-RBS-FMO-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4186
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2608
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. All codons were optimized for E. coli<.i> based on <i>E. coli codon bias.

2. Transformed and expressed in E. coli DH5α ΔTnaA to negate influence of endogenous TnaA in measurements.


Source

Tryptophanase (TnaA) is from E. coli and flavin-containing monooxygenase (FMO) is from Methylophaga aminisulfidivorans.

References